Polymerase Chain Reaction-based Detection of Cotton Leaf Curl and Other Whitefly-transmitted Geminiviruses from Sindh

Cotton Leaf Curl Disease, an Emerging Whitefly Transmissible Begomovirus Complex

In recent years leaf curl disease of cotton has become a major limiting factor in the production of cotton, which is an important fiber cash crop. In the last two decades or more, leaf curl disease in cotton took on epidemic proportions in Pakistan. In the early 1990’s cotton leaf curl disease (CLCuD) appeared in the bordering parts of India in the states of Rajasthan and Punjab and spread to e...

Nucleotide sequences from tomato leaf curl viruses from different countries: evidence for three geographically separate branches in evolution of the coat protein of whitefly-transmitted geminiviruses.

The coat protein (CP) gene-containing circular DNA molecule of an isolate of tomato leaf curl geminivirus (ITmLCV; 2749 nt) obtained from southern India, and the CP genes of tomato yellow leaf curl geminivirus isolates from Nigeria and two regions of Saudia Arabia were sequenced. ITmLCV DNA had the same arrangement of ORFs, and the same pattern of repeats in the large intergenic region as is fo...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Conventional breeding for resistance to whitefly-transmitted geminiviruses

The whitefly Bemisia tabaci Genn. is a pervasive pest and vector of plant viruses. The existence of B. tabaci biotypes and numerous whitefly-transmitted geminiviruses (begomoviruses) affecting food and industrial crops, has become a major constraint to agricultural development in tropical and subtropical regions of the world. The predominant whitefly and begomovirus method of control has been t...

1396 Cotton leaf curl disease

Cotton leaf curl disease is the major biotic constraint to cotton production in Pakistan and India.The disease causes unusual symptoms that include vein swelling, enations and the formation of leaf-like outgrowths. Cotton production during the 1990s was severely curtailed by CLCuD across the sub-continent. This stimulated interest in determining the aetiology and generating resistant varieties....